Antibodies, Assay Kits, cDNA, Cell-to-cell contact with hepatitis C virus-infected cells reduces functional capacity of natural killer cells, Chronic shift-lag alters the circadian clock of NK cells and promotes lung cancer growth in rats, Clia Kits, Culture Cells, Delineation and Modulation of the Natural Killer Cell Transcriptome in Rhesus Macaques During ZIKV and SIV Infections., Devices, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Equipments, Functions of natural killer cells, Gels, General, Healthy Neonates Possess a CD56-Negative NK Cell Population with Reduced Anti-Viral Activity, Human mesenchymal stem cells modulate allogeneic immune cell responses, Human NK cells kill resting but not activated microglia via NKG2D- and NKp46-mediated recognition, Isotypes, Medium & Serums, Overview of Strategies to Improve Therapy against Tumors Using Natural Killer Cell., Panel, Particles, Pcr Kits, Peptides, Reagents, Ria Kits, RNA, TRP Channels as Interior Designers: Remodeling the Endolysosomal Compartment in Natural Killer Cells., Vector & Virus

Host genetic control of natural killer cell diversity revealed in the Collaborative Cross

Natural killer (NK) cells are innate effectors armed with cytotoxic and cytokine-secreting capacities whose spontaneous antitumor exercise is vital to quite a few immunotherapeutic methods. However, present mouse fashions fail to reflect the in depth immune system variation that exists in the human inhabitants which can impression on NK cell-based therapies. We carried out a complete profiling of NK cells in the Collaborative Cross (CC), a set of novel recombinant inbred mouse strains whose genetic diversity matches that of people, thereby offering a novel and extremely various small animal mannequin for the research of immune variation.

We exhibit that NK cells from CC strains displayed a breadth of phenotypic and useful variation reminiscent of that reported for people on the subject of cell numbers, key marker expression, and useful capacities. We took benefit of the huge genetic diversity of the CC and recognized 9 genomic loci via quantitative trait locus mapping driving these phenotypic variations. SNP haplotype patterns and variant impact analyses recognized candidate genes related to lung NK cell numbers, frequencies of CD94+ NK cells, and expression ranges of NKp46.

Thus, we exhibit that the CC represents an impressive useful resource to check NK cell diversity and its regulation by host genetics.Natural killer (NK) cells, as a possible supply for off-the-shelf cell remedy, assault tumor cells with low danger of extreme cytokine launch syndrome (CRS) or graft-versus-host illness (GvHD). Fcγ receptor IIIA, also referred to as CD16, additional confers NK cells with antibody-dependent cell-mediated cytotoxicity (ADCC), one mechanism of motion of antibody-based immunotherapy.

Here, we set up a novel human NK cell line, oNK-1, endogenously expressing CD16 together with excessive ranges of NK activation markers and low ranges of NK inhibitory markers. The long-term growth and CD16 expression of oNK-1 cells had been demonstrated. Furthermore, oNK-1 cells elicit superior cytotoxicity in opposition to most cancers cells than major NK cells. In conclusion, this research means that endogenous CD16-expressing oNK-1 has the potential to develop an efficient NK-based remedy.

Core 2 β1,6-N-acetylglucosaminyltransferases speed up the escape of choriocarcinoma from natural killer cell immunity

 

Hyperglycosylated human chorionic gonadotropin (H-hCG) is secreted from choriocarcinoma and incorporates a core2 O-glycan shaped by core2 β1,6-N-acetylglucosaminyl transferase (C2GnT). Choriocarcinoma is taken into account immunogenic as it’s gestational and incorporates paternal chromosomal elements. Here we examined the perform of C2GnT in the evasion of choriocarcinoma cells from natural killer (NK) cell-mediating killing. We decided that C2GnT is very expressed in malignant gestational trophoblastic neoplasms.

C2GnT KO downregulates core2 O-glycan expression in choriocarcinoma cells, that are extra effectively killed by NK cells than control cells. C2GnT KO cell containing tumor necrosis factor-related apoptosis inducing ligand have decrease viability than control cells. Additionally, poly-N-acetyllactosamine in core2 branched oligosaccharides on MHC class I-related chain A (MICA) and mucin1 (MUC1) is considerably decreased in C2GnT KO cells. Meanwhile, the cumulative survival price of nude mice inoculated with C2GnT KO tumors was greater than that of the control group. These findings counsel that choriocarcinoma cells might escape NK cell-mediated killing by way of glycosylation of MICA and MUC1.

Invariant Natural Killer T (iNKT) cells are innate-like T Lymphocytes expressing a conserved semi-invariant T cell receptor (TCR) particular for self or microbial lipid antigens introduced by the non-polymorphic MHC class I-related molecule CD1d. Preclinical and medical research assist a task for iNKT cells in most cancers, autoimmunity and infectious ailments. iNKT cells are very conserved all through species and their investigation has been facilitated by mouse fashions, together with CD1d-deficient or iNKT-deficient mice, and the chance to unequivocally detect them in mice and males with CD1d tetramers or mAbs particular for the semi-invariant TCR.

However, iNKT cells are uncommon they usually must be expanded to succeed in manageable numbers for any research. Because the era of major mouse iNKT cell line in vitro has confirmed troublesome, now we have arrange a sturdy protocol to purify and increase splenic iNKT cells from the iVα14-Jα18 transgenic mice (iVα14Tg), in which iNKT cells are 30 instances extra frequent. We present right here that major splenic iVα14Tg iNKT cells could be enriched via an immunomagnetic separation course of, yielding about 95-98% pure iNKT cells.

Host genetic control of natural killer cell diversity revealed in the Collaborative Cross

Leukemia Inhibitory Factor Suppresses NKG2D mRNA Expression and Presentation on Human Natural Killer Cells

Leukemia inhibitory issue (LIF) is a multi-functional cytokine secreted from cells corresponding to lymphocytes and hepatocytes. This research aimed to judge the impact of LIF on natural killer group 2 member D (NKG2D) receptors’ expression and presentation on natural killer (NK) cells. For this function, peripheral blood mononuclear cells taken from four younger male wholesome blood donors had been remoted and the impact of LIF (25 ng/mL) after 12, 24, and 48 hours of incubation, on NKG2D receptors expression and presentation was investigated utilizing circulation cytometry and real-time-polymerase chain response (PCR).

10 mm Diameter Solid Titanium Tip

0-120-0009 10 mm
EUR 504

13 mm Diameter Tapped Titanium Tip

0-120-0010 13 mm
EUR 595

13 mm Diameter Solid Titanium Tip

0-120-0011 13 mm
EUR 533

19 mm Diameter Tapped Titanium Tip

0-120-0012 19 mm
EUR 639

19 mm Diameter Solid Titanium Tip

0-120-0013 19 mm
EUR 570

25 mm Diameter Tapped Titanium Tip

0-120-0014 25 mm
EUR 682

25 mm Diameter Solid Titanium Tip

0-120-0015 25 mm
EUR 614

Flat Replaceable Tips 13 mm Diameter Flat Titanium Tip

0-120-0016 13 mm
EUR 86
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Flat Replaceable Tips 19 mm Diameter Flat Titanium Tip

0-120-0017 19 mm
EUR 92
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip

0-120-0018 25 mm
EUR 100
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Titanium Cup Tip, 250 ml

0-120-0019 250 ml
EUR 1377
Description: includes Interface Washers

Microtube Tray, 8 Position (for 250 ml Cup Tip)

0-120-0021 each
EUR 113
Description: includes Interface Washers

Continuous Flow Chamber

0-120-0026 each
EUR 774
Description: includes Interface Washers

Interface Washers 1.5” Diameter, 5/Pkg

0-120-003 1.5'' Diameter
EUR 31

13 mm Diameter Solid Titanium Extender Tip

0-120-0032 13 mm
EUR 301

13 mm Diameter Tapped Titanium Extender Tip

0-120-0033 13 mm
EUR 362

19 mm Diameter Solid Titanium Extender Tip

0-120-0034 19 mm
EUR 315

19 mm Diameter Tapped Titanium Extender Tip

0-120-0035 19 mm
EUR 375

25 mm Diameter Solid Titanium Extender Tip

0-120-0036 25 mm
EUR 328

25 mm Diameter Tapped Titanium Extender Tip

0-120-0037 25 mm
EUR 389

KoldPod, 1.5ml Micro Tube

0-120-0038 1.5 ml
EUR 152
Description: Thermo conductive tube pods

KoldPod, 15ml Conical Tube

0-120-0039 15 ml
EUR 275
Description: Thermo conductive tube pods

KoldPod, 50ml Conical Tube

0-120-0040 50 ml
EUR 290
Description: Thermo conductive tube pods

Model 150 V/T Ultrasonic Homogenizer

0-121-0002 210-240V/50-60Hz
EUR 2920
Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing.

Model 300 V/T Ultrasonic Homogenizer

0-122-0002 210-240V/50-60Hz
EUR 3520
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample.

SONABOZ Sound Abating Chamber

0-125-0001 each
EUR 1020
Description: Reduces cavitational sound emitted during processing.

Model 3000 Ultrasonic Homogenizer

0-127-0002 210-240V/50-60Hz
EUR 4120
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample.

Model 3000MP Ultrasonic Homogenizer

0-128-0002 210-240V/50-60Hz
EUR 4720
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing.

OMNICON® Zone Reader, 210-240V/50-60Hz

0-131-0002 210-240V/50-60Hz
EUR 35200
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media.

OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz

0-134-0002 210-240V/50-60Hz
EUR 32200
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample.

Peni Cylinder Dispenser with Manual Hopper

0-144-0002 each
EUR 5406
Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish.

Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz

0-144-0003 100-240V/50-60Hz
EUR 6254
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection.

Stainless Steel Peni Cylinder with Flat Face

0-144-0005 6mm I.D. x 8mm O.D. x 10mm Long
EUR 399
Description: sold in packages of 100 pieces

Stainless Steel Peni Cylinder with Chamfered Face

0-144-0006 6mm I.D. x 8mm O.D. x 10mm Long
EUR 412
Description: sold in packages of 100 pieces

Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA)

000-CUS Custom
EUR 602

Alpha-Bungarotoxin, CF®405S, 500 ug

00002 1UG
EUR 527
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-bungarotoxin, CF405s

00002 500uG
EUR 594
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF®405S, 500 ug

00002-1 EA
EUR 527

Alpha-Bungarotoxin, CF®405S 100ug

00002-100ug 100uG
EUR 132
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®680R, 500 ug

9-00003
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®680R, 500 ug

00003-1 EA
EUR 527

Alpha-Bungarotoxin, CF®680R 100ug

9-00003
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®640R, 500 ug

9-00004
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®640R, 500 ug

00004-1 EA
EUR 527

Alpha-Bungarotoxin, CF®640R 100ug

9-00004
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®488A, 500 ug

9-00005
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®488A, 500 ug

00005-1 EA
EUR 527

Alpha-Bungarotoxin CF®488A 100ug

9-00005
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®568, 500 ug

9-00006
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®568, 500 ug

00006-1 EA
EUR 527

Alpha-Bungarotoxin, CF®568 100ug

9-00006
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®594, 500 ug

9-00007
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®594, 500 ug

00007-1 EA
EUR 527

Alpha-Bungarotoxin CF®594 100ug

9-00007
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®633, 500 ug

9-00009
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®633, 500 ug

00009-1 EA
EUR 527

Alpha-Bungarotoxin, CF®633 100ug

9-00009
  • Ask for price
  • Ask for price
  • 500uG
  • 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, 1 mg

00010-1 1MG
EUR 193
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, 1 mg

00010-1-1 EA
EUR 193

Fluorescein-Alpha-Bungarotoxin, 500 ug

00011 500uG
EUR 376
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Fluorescein-Alpha-Bungarotoxin, 500 ug

00011-1 EA
EUR 376

Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug

00012 500uG
EUR 394
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug

00012-1 EA
EUR 394

Fluorescein-alpha-bungarotoxin, 10x50ug

00013 10ST
EUR 436
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Fluorescein-alpha-bungarotoxin, 10x50ug

00013-1 EA
EUR 436

Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug

00014 10ST
EUR 494
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug

00014-1 EA
EUR 494

Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug

00015 500uG
EUR 494
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug

00015-1 EA
EUR 494

Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug

00016 10ST
EUR 560
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug

00016-1 EA
EUR 560

Biotin-XX-A-Bungarotoxin, 500 ug

00017 500uG
EUR 455
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Biotin-XX-A-Bungarotoxin, 500 ug

00017-1 EA
EUR 455

Alpha-Bungarotoxin, CF®555, 500 ug

9-00018
  • Ask for price
  • Ask for price
  • 500uG
  • 100ug
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®555, 500 ug

00018-1 EA
EUR 527

Alpha-Bungarotoxin, CF®555 100ug

9-00018
  • Ask for price
  • Ask for price
  • 500uG
  • 100ug
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Acrylamide, Chemzymes Ultra Pure®

00019-100 100g
EUR 91
Description: 79-06-1

Acrylamide, Chemzymes Ultra Pure®

00019-500 500g
EUR 241
Description: 79-06-1

Biotin-cAMP, 1 mg

00020 1MG
EUR 298
Description: N/A

Biotin-cAMP, 1 mg

00020-1 20ST
EUR 298
Description: N/A

Biotin-cAMP, 50 ug

00020-1-1 EA
EUR 414

Biotin-cGMP, 1 mg

00021 1MG
EUR 331
Description: N/A

Biotin-cGMP, 1 mg

00021-1 20ST
EUR 331
Description: N/A

Biotin-cGMP, 20x50 ug

00021-1-1 EA
EUR 447

Cyanine 644-cAMP, 1 mg

00022 1MG
EUR 496
Description: N/A

Cyanine 644-cAMP, 1 mg

00022-1 20ST
EUR 496
Description: N/A

Cyanine 644-cAMP, 20x50 ug

00022-1-1 EA
EUR 647

Fluorescein Methotrexate, Triammonium Salt, 1 mg

00023 1MG
EUR 285
Description: N/A

Fluorescein Methotrexate, Triammonium Salt, 1 mg

00023-1 EA
EUR 285

Staurosporine

00025 100uG
EUR 100
Description: N/A

Staurosporine

00025-1 EA
EUR 100

Alpha-Bungarotoxin, CF®543, 500 ug

00026 500uG
EUR 527
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®543, 500 ug

00026-1 EA
EUR 527

Alpha-Bungarotoxin, CF®543, 100 ug

00026-100ug 1UG
EUR 132
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Rhodamine Phalloidin 300U

00027 300
EUR 364
Description: N/A

Rhodamine Phalloidin 300U

00027-1 EA
EUR 364

Biotin-XX-Phalloidin

00028 100U
EUR 466
Description: N/A

Biotin-XX-Phalloidin

00028-1 EA
EUR 466

Fluorescein-Phalloidin

00030 300U
EUR 364
Description: N/A

Fluorescein-Phalloidin

00030-1 EA
EUR 364

Rhodamine 110 Phalloidin

00032 300ST
EUR 364
Description: N/A

Rhodamine 110 Phalloidin

00032-1 EA
EUR 364

Sulforhodamine 101 (Texas Red®) Phalloidin

00033 300EU
EUR 364
Description: N/A

Sulforhodamine 101 (Texas Red®) Phalloidin

00033-1 EA
EUR 364

Phalloidin, CF®405M

00034 300U
EUR 482
Description: N/A

Phalloidin, CF®405M

00034-1 EA
EUR 482

Phalloidin, CF®405M

00034-T 50U
EUR 101
Description: N/A

Phalloidin, CF®405M

00034-T-1 EA
EUR 101

CF®488A-cAMP

00036 100ug
EUR 136
Description: N/A

CF®488A-cAMP

00036-1 EA
EUR 136

CF®640R-cAMP

00037 100ug
EUR 136
Description: N/A

CF®640R-cAMP

00037-1 EA
EUR 136

Phalloidin, CF555

00040 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF®647, 300 U

00041 300U
EUR 482
Description: N/A

Phalloidin, CF®647, 300 U

00041-1 EA
EUR 482

Phalloidin, CF®647, 50 U

00041-T 50U
EUR 101
Description: N/A

Phalloidin, CF®647, 50 U

00041-T-1 EA
EUR 101

Phalloidin, CF®488A, 300 U

00042 300U
EUR 482
Description: N/A

Phalloidin, CF®488A, 300 U

00042-1 EA
EUR 482

Phalloidin, CF®488A, 50 U

00042-T 50U
EUR 101
Description: N/A

Phalloidin, CF®488A, 50 U

00042-T-1 EA
EUR 101

Phalloidin, CF®543, 300 U

00043 300U
EUR 482
Description: N/A

Phalloidin, CF®543, 300 U

00043-1 EA
EUR 482

Phalloidin, CF®543, 50 U

00043-T 50U
EUR 101
Description: N/A

Phalloidin, CF®543, 50 U

00043-T-1 EA
EUR 101

Phalloidin, CF®568, 300 U

00044 300U
EUR 482
Description: N/A

Phalloidin, CF®568, 300 U

00044-1 EA
EUR 482

Phalloidin, CF®568, 50 U

00044-T 50U
EUR 101
Description: N/A

Phalloidin, CF®568, 50 U

00044-T-1 EA
EUR 101

Phalloidin, CF®594, 300 U

00045 300U
EUR 482
Description: N/A

Phalloidin, CF®594, 300 U

00045-1 EA
EUR 482

Phalloidin, CF®594, 50 U

00045-T 50U
EUR 101
Description: N/A

Phalloidin, CF®594, 50 U

00045-T-1 EA
EUR 101

Phalloidin, CF®633, 300 U

00046 300U
EUR 482
Description: N/A

Phalloidin, CF®633, 300 U

00046-1 EA
EUR 482

Phalloidin, CF®633, 50 U

00046-T 50U
EUR 101
Description: N/A

Phalloidin, CF®633, 50 U

00046-T-1 EA
EUR 101

Phalloidin, CF®660R, 300 U

00047 300U
EUR 482
Description: N/A

Phalloidin, CF®660R, 300 U

00047-1 EA
EUR 482

Phalloidin, CF®660R, 50 U

00047-T 50U
EUR 101
Description: N/A

Phalloidin, CF®660R, 50 U

00047-T-1 EA
EUR 101

All of the steps of the experiment had been carried out in duplicate. After intervals of 12, 24, and 48 hours, LIF decreased each the expression and presentation of the NKG2D receptor on NK cells. The outcomes counsel that this cytokine has a direct modulating exercise on the physique’s immune response via suppression of NKG2D receptor expression and presentation on NK cells.